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During Wednesday's hearing at the High Court in Kampala witnesses in court said Besigye repeatedly shouted "stop" about his legal representation before collapsing.
"Before he collapsed, he cried out that he was being injured," his wife posted, adding that Besigye's sister Olive Kobusingye, who is a doctor, was in court but was not allowed to attend to him.
He was transferred to Mulago National Referral Hospital, Uganda's largest public hospital, but was refusing treatment from government medical personnel.
"He will not allow any government official to touch him," Byanyima said.
His wife demanded that Besigye be allowed to receive treatment from his personal physician at a private clinic rather than at a government health facility.
The ICU wing where Besigye is being treated is guarded by heavily armed security officers. Reports say his sister, who is also his personal doctor, has now been allowed to see him.
Besigye's main lawyer, Erias Lukwago, is in jail after being seized by the army last month.
His other lead lawyer, Kenyan Martha Karua, was barred from entering the country when she flew in to represent him last month.
Besigye's son, Adam Ampa, has appealed to President Museveni and his son, Gen Muhoozi Kainerugaba, who is Uganda's army chief, to show mercy by releasing his father on humanitarian grounds.
"Regardless of politics, no son should have to witness his father reach such a state while standing before the very law he served and dedicated his life," Ampa wrote in an open letter shared on social media on Wednesday.
Bobi Wine, a Ugandan opposition politician who fled the country fearing for his life after contesting the January presidential election, said Besigye's appearance in court while clearly unwell was a "shocking violation of his most basic rights".
Besigye once served as Museveni's personal doctor before breaking ranks with him in 1999.
He has been less active in politics in recent years, and did not contest the 2021 election.
